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R&D Technician II - NGS Molecular
Location: Lenexa, KS
| Job Summary and Purpose: |
Apply general molecular biology methods to ensure timely processing and identification of bacterial and viral samples. Complete laboratory services to internal and external customers when requested. Participate in R&D projects as assigned.
| Responsibilities and Key Duties: |
Molecular Assays - use and development
* Apply general molecular biology methods to identify relevant animal pathogens (nucleic acid extraction, PCR, RT-PCR, qPCR, RT-qPCR, Sanger DNA sequencing)
* Perform Next Generation Sequencing using long read technologies (Oxford Nanopore Technology) and short read technologies (Illumina)
* Work with various molecular instruments (nucleic acid extraction robots, conventional PCR thermocyclers, real-time PCR thermocyclers, mixers, incubators, freezers)
* Coordinate with internal departments to provide laboratory services when requested
* Investigate expanding capabilities of services offered internally and externally with new tech research
* Learn to operate, validate, and incorporate new technology into internal/external repertoire as it is acquired and installed
* Prepare reports as needed for customers
* Prepare project reports
* Participate in the design, optimization, validation of new PCR and sequencing methods
* Report progress to leadership team
Bioinformatics
* Analyze Sanger sequencing data
* Use commercial software (Geneious, Bionumerics) and open source software to analyze NGS data
* Present data and reports with relevant sequence analyses (phylogenetic trees, heat maps, SNP analysis)
* Teamwork with other R&D groups, Production Departments and Scientific Support/Product Support Personnel
* Assay development in support of new products
* Assist in projects related to Scientific Support/Product Support
* Contribute to training materials, protocols, and reports
* Provide training to new technicians, students, and interns
* Assist with animal studies
Documentation and Internal Quality Assurance
* Maintain sample and test database
* Maintain and review complete and accurate bench records following appropriate guidelines
* Contribute to and/or write SOPs and when necessary, develop record forms
Laboratory Maintenance
* Organize and maintain inventory records for freezers and contents
* Maintain laboratory and equipment cleaning schedules
* Maintain laboratory supplies, order reagents
* Adhere to appropriate safety requirements and protocols
| Core Competencies: |
* Shape solutions out of complexity - Is open and curious of other perspectives
* Client focus - Has in mind client's satisfaction
* Collaborate with empathy - Has a positive attitude towards collaboration
* Engage and develop - Clarifies short-term directions
* Drive ambition and accountability - Proactively manages own and others' work
* Influence others - Communicates transparently
| Technical / Functional Competencies: |
* Basic computer skills
o Operating systems - Windows, Mac, and/or Linux
o Microsoft Office or equivalent
o Bioinformatic analyses using Geneious Prime
* Basic microbiology skills (bacteriology and virology)
* Advanced molecular biology skills
* Knowledgeable about Biosafety in Microbiological and Biomedical Laboratories (BMBL)
* Basic knowledge of animal infectious diseases
* Good communication skills - both written and verbal
| Qualifications: |
Education - master's in microbiology or biology or equivalent preferred or BA/BS in science related field, or equivalent with 1-3 years of relevant experience
Work Experience - 1-3 years of relevant experience:
* Advanced knowledge with molecular biology techniques is a necessity
* Intermediate knowledge in microbiology and chemistry a plus
* Assay development and validation (e.g. PCR, RT-PCR, qPCR, and RT-qPCR) a plus
* Animal bacterial and/or virology disease model development a plus
| Physical Requirements: |
* Able to read, write legibly, and communicate in English
* Ability to lift up to 30 pounds
* Frequent standing for long periods of time
* Visual Inspection
| Working Conditions: |
* N/A
| Travel Required: |
* N/A
This job description is intended to convey information essential to understanding the scope of the job and general nature and level of work performed by job holders within this job. This job description is not intended to be an exhaustive list of qualifications, skills, duties and responsibilities or working conditions associated with the job. This job description is not an employment contract. Ceva reserves the right to modify job duties and/or job descriptions at any time to meet the needs of the business.
*VEVRAA Federal Contractor
**Ceva is an equal opportunity employer and all qualified applicants will be considered for employment without regard to race, color, religion, sex, national origin, age, genetic information, actual or perceived sexual orientation, gender identity, disability status, protected veteran status, or any other characteristic protected by law. Ceva encourages men and women, members of all racial and ethnic groups, individuals with disabilities and veterans to apply.
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